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( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and <t>NT2</t> cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.
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( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and <t>NT2</t> cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.
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( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and <t>NT2</t> cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.
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( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and <t>NT2</t> cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.
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( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and <t>NT2</t> cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.
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( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: ( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

Article Snippet: We purchased astrocytoma (cat# CCF-STTG1), HMC3 (cat # CRL-3304), SH-SY5Y (cat # CRL2266), and Ntera-2 (NT2) cells (cat # CRL-1973) from ATCC (Manassas, VA, USA) as cellular models of astrocytes, microglia, neuroblastoma, and neurons, respectively.

Techniques: Western Blot, Expressing, Staining